Search results for " human pluripotent stem cells"

showing 2 items of 2 documents

Quantitative mass spectrometry for human melanocortin peptides in vitro and in vivo suggests prominent roles for β-MSH and desacetyl α-MSH in energy …

2018

Objective The lack of pro-opiomelanocortin (POMC)-derived melanocortin peptides results in hypoadrenalism and severe obesity in both humans and rodents that is treatable with synthetic melanocortins. However, there are significant differences in POMC processing between humans and rodents, and little is known about the relative physiological importance of POMC products in the human brain. The aim of this study was to determine which POMC-derived peptides are present in the human brain, to establish their relative concentrations, and to test if their production is dynamically regulated. Methods We analysed both fresh post-mortem human hypothalamic tissue and hypothalamic neurons derived from …

MalePluripotent Stem CellsLeptinlcsh:Internal medicineendocrine systemhPSC human pluripotent stem cellsPro-Opiomelanocortin[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/NeurobiologyHypothalamusMass SpectrometryTandem Mass Spectrometry[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]beta-MSHHomeostasisHumansHuman pluripotent stem cellObesitylcsh:RC31-1245MSHNeuronsintegumentary systemReceptors MelanocortinLC-MS/MS liquid chromatography tandem mass spectrometryNeuropeptidesdigestive oral and skin physiologyPOMCPVH the paraventricular nucleus of the hypothalamusCTX cerebral cortexMelanocortinsNeuropeptidealpha-MSHOriginal ArticleFemalehormones hormone substitutes and hormone antagonistsChromatography Liquid
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Poly-l-Lactic Acid Nanofiber-Polyamidoamine Hydrogel Composites: Preparation, Properties, and Preliminary Evaluation as Scaffolds for Human Pluripote…

2016

Electrospun poly-l-lactic acid (PLLA) nanofiber mats carrying surface amine groups, previously introduced by nitrogen atmospheric pressure nonequilibrium plasma, are embedded into aqueous solutions of oligomeric acrylamide-end capped AGMA1, a biocompatible polyamidoamine with arg-gly-asp (RGD)-reminiscent repeating units. The resultant mixture is finally cured giving PLLA-AGMA1 hydrogel composites that absorb large amounts of water and, in the swollen state, are translucent, soft, and pliable, yet as strong as the parent PLLA mat. They do not split apart from each other when swollen in water and remain highly flexible and resistant, since the hydrogel portion is covalently grafted onto the …

Materials Chemistry2506 Metals and AlloysPluripotent Stem CellsAgmatinePolymers and PlasticsDouble bondpolyamidoaminesPolyestersCell Culture TechniquesNanofibersBioengineering02 engineering and technology010402 general chemistry01 natural sciencesBiomaterialsPolyamidoaminePolyaminesMaterials ChemistryHydrogel compositehuman pluripotent stem cellHumansatmospheric pressure nonequilibrium plasmaInduced pluripotent stem cellatmospheric pressure nonequilibrium plasma; electrospun poly-l-lactic nanofibers; human pluripotent stem cells; poly-l-lactic acid-AGMA1 hydrogel composites; polyamidoamines; biotechnology; bioengineering; biomaterials; polymers and plastics; materials chemistry2506 metals and aloyschemistry.chemical_classificationAddition reactionPolymers and PlasticAqueous solutionTissue ScaffoldsHydrogels021001 nanoscience & nanotechnologyBiomaterial0104 chemical sciencesChemical engineeringchemistryCovalent bondNanofiberelectrospun poly-l-lactic nanofiberpoly-l-lactic acid-AGMA1 hydrogel compositeAmine gas treating0210 nano-technologyBiotechnology
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